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PMSF is a inhibitor of serine proteases and acetylcholinesterase. It inhibits proteases such as
chymotrypsin, trypsin, thrombin and thiol proteases such as papain. PMSF inhibits serine proteases by
sulfonating serine residues at the active site.
Storage/Stability:
When stored as indicated, PMSF has a shelf-life of three years..
Preparation Instructions:
PMSF is soluble in anhydrous isopropanol at 35 mg/ml with heating, resulting in a clear to very slightly
hazy, colorless to faint yellow solution.
Recommended stock solution: 100 mM in anhydrous isopropanol or anhydrous (100%, not 95%) ethanol.
A 200 mM solution in dry solvent is stable for at least 9 months at 2-8° C.
PMSF is very unstable in the presence of water. The half-life of aqueous PMSF at 25° C at pH 7.0, 7.5,
and 8.0 is 110, 55, and 35 minute respectively.
Precautions and Disclaimer：
PMSF is considered a highly toxic cholinesterase inhibitor.